Detection of the intergenic region III and multigene family 505 9R and 10R-V variant of African swine fever virus genotype II on pig farms in South Korea

Figures

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  Fig. 1. Phylogenetic trees of eight African swine fever virus (ASFV) isolates from pig farms collected between July 2023 and July 2024, constructed using partial B646L (p72), E183L (p54), and EP402R (CD2v) sequences (red circles). (a and b) p72 and p54 genotypes by phylogenetic analysis using the neighbor-joining method. (c) CD2v serogroup by phylogenetic analysis using the Kimura 2-parameter model

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  Fig. 2. Analysis of intergenic regions (IGR) between the I73R and I329L genes (a) and between multigene families 505 9R and 10R (b) of eight African swine fever virus isolates. (a) One isolate, Korea/Pig/Andong/2024, was classified as IGR-III, with four copies of the 10-nucleotide tandem repeat sequence (GAATATATAG), while the other seven isolates were IGR-Ⅱ (red circles). (b) Eight MGF_505 9R/10R (MGF) variants that differ in the number of repetitions and types of tandem repeat sequences. Four ASFV isolates originating from pig facilities in the east-central region were of the MGF-Ⅴ variant tape. The figure was modified based on information presented previously [20]

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  Fig. 3. Map of South Korea showing the locations where ASFV was detected on 44 pig farms (circles) and in 4143 wild boars (gray triangles) between September 2019 and July 2024. In 2024, a variant with IGR-III and multigene family 505_9R/10R (MGF)-Ⅴ was detected on a pig farm in Andong City (red circle). At three pig facilities located within a 50-km radius of the pig farm in Andong City, IGR-Ⅱ-MGF-Ⅴ variants were found (yellow circles). The viruses from the remaining 40 pig farms were classified as IGR-Ⅱ-MGF-I (blue circles), except for one with IGR-I-MGF-V (green circle) near the northwestern border