Key result
The novel CRISPR-Cas12a based assay detected African swine fever virus with a limit of detection of 1.16 copies/μl, demonstrating approximately ten times higher sensitivity than commercial qPCR.
Why the study?
Because no treatment or effective vaccine for African swine fever virus is currently available, a rapid and highly sensitive diagnostic was urgently needed to curb its spread.
Does a CRISPR-Cas12a based assay improve the detection sensitivity of African swine fever virus compared to standard qPCR in porcine samples?
Comparison
Novel CRISPR-Cas12a based assay vs commercial qPCR kit and OIE-recommended qPCR
Design
Diagnostic assay development and validation study
Authors
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CRISPR-Cas12a assay enables adaptable ASFV detection; leaves open real-world sensitivity across field samples and genotypes.
Does a CRISPR-Cas12a based assay improve the detection sensitivity of African swine fever virus compared to standard qPCR in porcine samples?
Absolute Event Rate: 1.16% vs 11.6%
The novel CRISPR-Cas12a based assay provides a highly sensitive, specific, and rapid method for detecting African swine fever virus, outperforming standard qPCR in limit of detection.
Wang et al. (2020) studied African swine fever virus (ASFV) (n=101). CRISPR-Cas12a based assay vs. Commercial qPCR kit and OIE-recommended qPCR was evaluated on Limit of detection (sensitivity). The novel CRISPR-Cas12a based assay detected African swine fever virus with a limit of detection of 1.16 copies/μl, demonstrating approximately ten times higher sensitivity than commercial qPCR.
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