Key result
A simple, sensitive, and rapid LC-MS/MS method was successfully developed and validated for the simultaneous quantification of propafenone and its two major metabolites in human plasma.
A rapid and sensitive LC-MS/MS method was successfully developed and validated for the simultaneous quantification of propafenone and its metabolites in human plasma for pharmacokinetic studies.
Validated LC-MS/MS assay enables PPF metabolite quantification in plasma; leaves open clinical TDM adoption pending further studies.
Objective: A simple, sensitive and rapid performance liquid chromatography/positive ion electrospray tandem mass spectrometry method was to be developed and validated for quantification of propafenone (PPF) and its two major metabolite 5-hydroxy propafenone (5-OHP) and N-depropyl propafenone (NDP) in human plasma.Methods: Liquid-liquid extraction (LLE) with ethyl acetate was used of extraction of plasma samples. The analytes were separated using an isocratic mixture of 0.1% formic acid/acetonitrile (20:80 v/v) on a reversed-phase column Hypurity Advance C18 50 x2.1 mm, 5µ and analysed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H] Ions. The m/z was 342.20/116.10 for propafenone, m/z 299.80/74.10 for N depropyl propafenone and m/z 358.30/98.10 for 5-hydroxy propfenone along with m/z 409.2/238.0 for Amlodipine as internal standard respectively.Results: The method had a short chromatographic run time of 1.5 min. The method exhibited a linear dynamic range over 5.11 to 1000.73 ng/ml for propafenone, 0.51 to 100.06 ng/ml for N-depropyl propafenone and 5.11 to 1001.64 ng/ml for 5-hydroxy propafenone respectively, in human plasma.Conclusion: The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetics, bioavailability and bioequivalence studies.
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Gupta et al. (2017) studied this question. LC-MS/MS method was evaluated on Method validation (linearity, precision, accuracy, recovery). A simple, sensitive, and rapid LC-MS/MS method was successfully developed and validated for the simultaneous quantification of propafenone and its two major metabolites in human plasma.
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