Key result
Lowering intracellular pH from 7.2 to 6.6 reduced the ROMK1 current by 80%, whereas extracellular acidification had no effect.
Why the study?
Does intracellular acidification inhibit the ROMK1 K+ channel expressed in Xenopus oocytes?
Population
Xenopus oocytes expressing cloned rat kidney K+ channel ROMK1
Comparison
Lowering external pH from 7.4 to 6.7 using… vs Baseline pH 7.4 or extracellular acidification…
Design
Preclinical
Authors
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May modulate renal K+ secretion via ROMK1; leaves open in vivo relevance in mammalian kidney.
Does intracellular acidification inhibit the ROMK1 K+ channel expressed in Xenopus oocytes?
Intracellular acidification strongly inhibits the ROMK1 potassium channel, suggesting a mechanism for pH-dependent regulation of renal potassium secretion.
Tsai et al. (1995) studied this question. Intracellular acidification vs. Extracellular acidification was evaluated on ROMK1 current. Lowering intracellular pH from 7.2 to 6.6 reduced the ROMK1 current by 80%, whereas extracellular acidification had no effect.
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