Key result
Application of ATP, ADP, or AMP to the cytoplasmic face of excised membrane patches from mouse kidney reduced the open-state probability of cation channels in a dose-dependent manner.
Population
Excised basolateral membrane patches from the thick ascending limb of Henle's loop of the mouse kidney
Comparison
Application of adenine nucleotides and analogues… vs Control or other purines/pyrimidines
Design
Preclinical
Authors
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Does not inform clinical practice; hypothesis-generating for nucleotide regulation of renal cation channels in vivo.
Adenine nucleotides (ATP, ADP, AMP) inhibit Ca2+-activated non-selective cation channels in the basolateral membrane of the mouse kidney thick ascending limb.
Paulais et al. (1989) studied this question. Adenine nucleotides (ATP, ADP, AMP) vs. Control was evaluated on Open-state probability of the channels (Po). Application of ATP, ADP, or AMP to the cytoplasmic face of excised membrane patches from mouse kidney reduced the open-state probability of cation channels in a dose-dependent manner.