Practical assay for determining residual sulfite of the wine in rapid detection or quantitative analysis

A colorimetric and ratiometric fluorescence probe FS-wine with near-infrared emission for rapid detection of sulfites was proposed and developed. It is evident that FS-wine could clarify the uncertain content of sulfites in some colorless or translucent wine within the rapid detection. In addition, a practical assay for determining residual sulfites (R2 > 0.99) was established with the variation of Em480 nm/Em650 nm (excited at 410 nm). The assay was effective in detecting sulfites in wine samples with the exclusion of potential interference. The results achieved using FS-wine were consistent with those obtained using the standard method (P > 0.05), with the spiked recovery rate ranging from 99.9% to 110.3%. In addition, the dominant residual sulfite could be confirmed as HSO3− in the wine samples with this assay, which was consistent with the interpretation supposed from the native chemical characterization of sulfites. These results affirmed that FS-wine could efficiently detect sulfites in the food to assure food safety when sulfites were used as preservation agent.