Expression characteristics and their functional role of IGFBP gene family in pan-cancer

Figures

• 

  Fig. 1. Expression profile of IGFBPs across different cancer types. A Expression of IGFBPs in different cancer and normal samples. The color in heatmap represents the log2 fold change value between cancer and normal. The blue color represents the low expression in cancer, whereas the red color represents the high expression in cancer. The * sign represents degree of statistical significance, * P < 0.05, ** P < 0.01, *** P < 0.001. B Expression of IGFBP1 in 10 types of cancers between cancer and normal tissues. C Expression of IGFBPs in various cancers from Oncomine database. The blue color represents the low expression, whereas the red color represents the high expression

• 

  Fig. 2. The expression profile of IGFBPs at cell and protein level. A Expression heatmap of IGFBPs in 6 cancer cell lines (breast, colorectal, gastric, kidney, liver and lung) from CCLE database. The blue color represents the low expression, whereas the red color represents the high expression. B Boxplot of IGFBPs expression in 6 cancer cell lines. C IGFBPs protein expression across various cancer types. The expression of each gene in each cancer was divided into four groups of high expression (red color), medium expression (orange color), low expression (yellow color) and not detected (grey color). D IGFBP6 protein expression in 16 cancer types based on immunohischemistry staining results from 'The Human Protein Atlas' database

• 

  Fig. 3. Correlation between IGFBPs expression and age of patients in pan-cancer. Blue bars represent younger age (< = 60), while red bars represent older age (> 60). * P < 0.05, ** P < 0.01, *** P < 0.001

• 

  Fig. 4. Correlation between IGFBPs expression and gender of patients in pan-cancer. Blue bars represent male, while red bars represent female. * P < 0.05, ** P < 0.01, *** P < 0.001

• 

  Fig. 5. Prognostic significance of IGFBP genes. A Correlation between IGFBPs expression and survival of different cancers. Red color represents high risk of death whereas blue color represents low risk of death. B, C Forest plot for the prognostic analysis of IGFBP2 and IGFBP7 across 33 cancer types

• 

  Fig. 6. Association of IGFBPs expression with cancer-related pathways. A Network displaying the correlation between IGFBPs expression and cancer-related pathways. The white nodes represent IGFBPs and grey nodes represent pathways. The red edges represent activated pathways and blue edges represent inhibited pathways. B The number of significantly correlated pathways in each individual IGFBPs. Red color represents activated pathways and blue color represents inhibited pathways. C Correlation between the expression of different IGFBPs. Red color represents positive correlation and blue color represents negative correlation

• 

  Fig. 7. The correlation and interaction analysis among IGFBPs. A IGFBPs expression in all cancer patients in TCGA. B The correlation among IGFBPs expression in all 33 types of cancer from TCGA. Red color represents positive correlation and blue color represents negative color (P < 0.05). C Interaction network of IGFBPs from GENEMANIA. D Protein–protein interaction network of IGFBPs from STRING. Red color represents high degree score and blue color represents low degree score

• 

  Fig. 8. The correlation among IGFBPs expression in different 9 types of cancer from TCGA

• 

  Fig. 9. Correlation between IGFBPs expression and (A) tumor mutational burden (TMB) as well as (B) microsatellite instability (MSI) in pan-cancer. * P < 0.05, ** P < 0.01, *** P < 0.001

• 

  Fig. 10. Correlation between IGFBPs expression and stemness score in pan-cancer. A Correlation between IGFBPs expression and DNAss. B Correlation between IGFBPs expression and RNAss. The red color represents positive correlations, whereas the green color represents negative correlations. The depth of the color represents the strength of the relationship. * P < 0.05, ** P < 0.01, *** P < 0.001. DNAss, DNA stemness score; RNAss, RNA stemness score

• 

  Fig. 11. Correlation between IGFBPs expression and tumor immune microenvironment in pan-cancer. A Correlation between IGFBPs expression and stromal scores. B Correlation between IGFBPs expression and immune scores. C Correlation between IGFBPs expression and ESTIMATE scores. The red color represents positive correlations, whereas the blue color represents negative correlations. The depth of the color represents the strength of the relationship. * P < 0.05, ** P < 0.01, *** P < 0.001

• 

  Fig. 12. Correlation between IGFBPs expression and immune cell infiltration. A Correlation between IGFBP1 expression and Dendritic cells activated infiltration level in KICH. B Correlation between IGFBP2 expression and Mast cells resting cell infiltration level in THYM. C Correlation between IGFBP3 expression and Macrophages M2 cell infiltration level in TGCT. D Correlation between IGFBP4 expression and Dendritic cells activated cell infiltration level in ACC. E Correlation between IGFBP5 expression and Macrophages M2 cell infiltration level in TGCT. F Correlation between IGFBP6 expression and T cells CD8 cell infiltration level in KICH. G Correlation between IGFBP7 expression and Macrophages M1 cell infiltration level in ACC. H Correlation between IGFBPL1 expression and Mast cells resting cell infiltration level in KIRP