Key result
The use of commercial bovine viral diarrhoea virus Ag-ELISA for detecting hairy shaker disease virus antigen in sheep is discussed to clarify its diagnostic utility.
The paper discusses the use of commercial BVD virus Ag-ELISA for detecting HSD virus antigen in sheep due to the lack of a commercially available HSD-specific ELISA.
Answers are grounded in this paper and cite their sources.
May offer interim diagnostic option for hairy shaker disease in sheep; leaves open validation of accuracy in ovine populations.
On several occasions over recent years, the Investigation and Diagnostic Centre Animal Health Laboratory (IDC-AHL) was approached by external clients to perform testing of serum samples from sheep for hairy shaker disease (HSD)/border disease by using the HSD ELISA for viral antigen detection (Ag-ELISA), rather than virus isolation. The reasons were that the Ag-ELISA is faster and less expensive, is more suitable for large numbers of samples, and is a prescribed test method for international trade (Nettleton and Willoughby 2008 Nettleton, PF and Willoughby, K. 2008. “Border disease”. In OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 963–973. Paris, , France: Organization International des Épizooties. [Google Scholar]). Because an HSD virus Ag-ELISA is currently not commercially available, clients suggested the use of a commercial bovine viral diarrhoea (BVD) virus Ag-ELISA for the detection of HSD virus antigen as an alternative, as this approach had been published before (Orr et al. 1993 Orr, MB, Horner, GW and Harkness, JW. 1993. A comparison of diagnostic tests for hairy shaker disease. New Zealand Veterinary Journal, 41: 211–211. [Taylor & Francis Online], [Web of Science ®] , [Google Scholar]; Schroeder et al. 2003 Schroeder, C, Nettleton, P, Toomik, R and Ballagi, A. 2003. “Current data on detection of BVDV and BDV infection using HerdChek BVDV antigen ELISAs”. In Proceedings of the 11th International Symposium of the World Association of Veterinary Laboratory, Diagnosticians and OIE Seminar on Biotechnology, Edited by: Thanawongnuwech, R and Ingkaninun, P. Thailand: P O21. Thai Association of Veterinary Laboratory Diagnosticians. In [Google Scholar]). Here, we would like to clarify the confusing situation regarding the use of BVD virus Ag-ELISA for the detection of HSD virus antigen. We discuss this approach in detail in the context of information gathered from various experts in the field.
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Kittelberger et al. (2008) conducted a letter in Hairy shaker disease (HSD)/border disease in sheep. Bovine viral diarrhoea (BVD) virus Ag-ELISA vs. Virus isolation was evaluated. The use of commercial bovine viral diarrhoea virus Ag-ELISA for detecting hairy shaker disease virus antigen in sheep is discussed to clarify its diagnostic utility.
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