Key result
Novel EQL.G3 qRT-PCR assay matches reference assays with ~100% positive agreement for detecting rotavirus strains.
The newly developed qRT-PCR assay provides a highly sensitive, specific, and rapid method for detecting and quantifying emerging equine-like G3 rotavirus strains in human stool samples.
May aid high-throughput screening for equine-like G3 rotavirus; leaves open routine diagnostic adoption pending clinical validation.
copies per reaction and a limit of detection of 227 copies. The percent positive agreement, percent negative agreement, and precision of our assay were 100.00%, 99.63%, and 100.00%, respectively. This assay can simultaneously detect and quantify the viral load for samples containing DS-1-like intergenogroup reassortant equine-like G3 strains with high sensitivity and specificity, faster turnaround time, and decreased cost. It will be valuable for high-throughput screening of stool samples collected to monitor equine-like G3 strain prevalence and circulation among human populations throughout the world.
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Katz et al. (2021) studied Group A rotavirus (equine-like G3 strains) (n=470). EQL.G3 qRT-PCR assay vs. Reference genotyping assays was evaluated on Percent positive agreement for detection of EQL.G3 genotype (95% CI 98.47-100.00). The novel EQL.G3 qRT-PCR assay demonstrated 100.00% positive agreement and 99.63% negative agreement for detecting rotavirus equine-like G3 strains compared to reference genotyping assays.
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