Knockdown of Telethonin Reduces Contractions and Provokes Aberrant Ca2+-waves in Human iPS Cell-induced Cardiomyocytes

Figures

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  Figure 1. The expressions of TCAP mRNA measured by the RT-qPCRs Relative mRNA expression levels normalized by the GAPDH were decreased in TCAP KD (n = 5) compared to WT cells (n = 4).

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  Figure 2. The expressions of telethonin measured by Western blotting (A) Representative data of Western blotting. Recombinant telethonin antigen was used as a positive control (PC). (B) Relative telethonin expressions normalized by the β-Actin were decreased to 47.1% in the TCAP KD (n = 5) compared to the WT cells (n = 5).

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  Figure 3. Representative motion vector waveforms obtained from contracting cardiomyocytes differentiated from TCAP WT and KD The vertical axis represents velocity (μm/s) and the horizontal axis represents time (s). The recording of the 10-s period of electrical stimulation (1-sec interval, 5 msec duration, 50 V) indicates that the biphasic waveforms represent myocardial contraction as the first peak (yellow plots), followed by relaxation as the second peak (blue plots).

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  Figure 4. Summary data of contractility parameters All contractility parameters were decreased in the KD-CMs (n = 54) compared to the WT-CMs (n = 24).

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  Figure 5. Representative trace of Ca2+-transients observed in iPSC-CMs The Ca2+-signals were recorded under 1 Hz field stimulation. Note that the aberrant Ca2+-waves persisted in the TCAP-KD CMs.