Key result
PCR rapidly resolves IL-6 gene alleles missed by conventional Southern analysis.
PCR provides a rapid, non-radioactive, and efficient method for detecting and sequencing highly polymorphic length variations in the AT-rich 3' flanking region of the IL6 gene.
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Offers faster IL-6 genotyping in research; leaves open any clinical role in cardiovascular risk assessment.
Bowcock et al. (1989) studied this question. Polymerase chain reaction (PCR) amplification vs. Conventional genomic blotting (Southern analysis) was evaluated on Detection and resolution of length polymorphisms in the 3' flanking region of the IL-6 gene. A PCR-based method successfully and rapidly amplified the AT-rich 3' flanking region of the interleukin-6 gene, resolving alleles that could not be typed by conventional Southern analysis.
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