Key result
The universal real-time fluorescence qPCR method achieved a limit of detection of 3.2 copies/μL and detected ASFV in 54.4% of suspected pig tissue samples, outperforming standard methods for weakly positive samples.
Why the study?
The study was conducted to establish a highly sensitive real-time fluorescence quantitative PCR method for universal testing of epidemic African swine fever virus strains.
Does the newly developed real-time fluorescence qPCR method improve the detection of epidemic ASFV strains compared to standard methods in pig samples?
Population
142 clinically suspected samples from China and imported goods
Comparison
Novel qPCR method vs standard OIE-recommended qPCR method and commercially available kit
Authors
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May aid ASFV surveillance in tested samples; leaves open need for independent validation before wider use.
Does the newly developed real-time fluorescence qPCR method improve the detection of epidemic ASFV strains compared to standard methods in pig samples?
Absolute Event Rate: 54.4% vs 52.9%
The novel real-time fluorescence qPCR method offers highly sensitive, universal detection of 24 ASFV genotypes, improving early diagnosis in weakly positive samples compared to standard methods.
Lv et al. (2021) studied African Swine Fever Virus (ASFV) (n=142). Universal real-time fluorescence qPCR method vs. OIE recommended qPCR method and commercially available kit was evaluated on Positive detection rate in clinically suspected pig tissue samples. The universal real-time fluorescence qPCR method achieved a limit of detection of 3.2 copies/μL and detected ASFV in 54.4% of suspected pig tissue samples, outperforming standard methods for weakly positive samples.
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