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December 10, 2025Frontiers in Veterinary ScienceOpen Access

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

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Authors

SGSilvia Dei GiudiciPBPiero BonelliMTMaria Giovanna Tilocca

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Overview

Comparison shows digital PCR and real time PCR methods provide reliable diagnosis for African Swine Fever Virus.

Key Points

  • This research aims to evaluate the performance of digital PCR relative to real time PCR for African Swine Fever virus detection.
  • Standardized droplet digital PCR protocols were developed
  • Compared analytical performance using WOAH-recommended primers
  • Analyzed clinical positive and negative samples alongside plasmid templates
  • Digital PCR demonstrated excellent linearity (R^2 = 0.999) across a wide range
  • Limits of detection were found to be comparable to real time PCR
  • Strong agreement between qPCR and ddPCR indicated reliable diagnosis for ASFV

Cite This Study

Giudici et al. (2025) studied this question.

synapsesocial.com/papers/69401d412d562116f28f83e2https://doi.org/10.3389/fvets.2025.1704297
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