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February 5, 2021Frontiers in Veterinary ScienceOpen Access

Nanofluidic chip digital PCR demonstrated a 91.30% total coincidence rate with OIE-approved quantitative PCR for detecting African Swine Fever Virus in clinical samples.

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Why the study?

To prevent and control African swine fever, which causes severe economic loss in the swine industry, reliable laboratory testing is required to diagnose suspected infections.

Population

69 clinical samples

Comparison

Nanofluidic chip digital PCR vs OIE-approved quantitative PCR and a commercial quantitative PCR kit

Design

Diagnostic validation study

Key result

Nanofluidic chip digital PCR demonstrated a 91.30% total coincidence rate with OIE-approved quantitative PCR for detecting African Swine Fever Virus in clinical samples.

Authors

JRJia RuiGZGaiping ZhangHLHongliang Liu

Discussion

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Member takes

Overview

May enable faster ASFV DNA quantification; leaves open diagnostic validation before field adoption.

Structured PICO

P
Population
69 clinical swine serum samples were tested to evaluate the diagnostic performance of a novel nanofluidic chip digital PCR for detecting African Swine Fever Virus.
I
Intervention
Nanofluidic chip digital PCR (cdPCR) targeting the ASFV B646L gene
C
Comparator
OIE-approved quantitative PCR (qPCR) and a commercial qPCR kit (VetMAX African Swine Fever Virus Detection Kit)
O
Outcome
Limit of detection, sensitivity, specificity, and coincidence rate for ASFV detection

Main Result

Effect estimate: Kappa 0.789

p-value: p=<0.0001

Nanofluidic chip digital PCR provides a highly sensitive and specific method for the absolute quantification of African Swine Fever Virus DNA in clinical samples.

Limitations

  • Specific equipment is required for nanofluidic cdPCR, making it hard to popularize and widely apply.
  • The specialized nanofluidic chip that accompanies the digital PCR platform is expensive.
  • cdPCR can only amplify a maximum of 24 samples in a single run, which is 72 samples fewer than qPCR.

Cite This Study

Rui et al. (2021) studied African Swine Fever Virus (ASFV) (n=69). Nanofluidic chip digital PCR (cdPCR) vs. OIE-approved quantitative PCR was evaluated on Total coincidence rate with OIE-approved qPCR (Kappa 0.789, p=<0.0001). Nanofluidic chip digital PCR demonstrated a 91.30% total coincidence rate with OIE-approved quantitative PCR for detecting African Swine Fever Virus in clinical samples.

synapsesocial.com/papers/6aa01e44fb5e17fb76d561aahttps://doi.org/10.3389/fvets.2020.621840
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus2025 · 2 citations
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  4. 4Triplex Crystal Digital PCR for the Detection and Differentiation of the Wild-Type Strain and the MGF505-2R and I177L Gene-Deleted Strain of African Swine Fever Virus2023 · 9 citations
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