Key result
HSV-1 amplicons suppress sodium currents by ~80% and reduce spike frequency in cultured neurons.
The 5dl1.2 helper virus used in HSV-1 amplicon preparations potently suppresses sodium currents in transduced neurons, indicating a significant unintended impact on neuronal physiology.
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May confound neuronal electrophysiology readouts in HSV-1 amplicon studies; leaves open need for helper-free vectors before in vivo translation.
White et al. (2002) studied this question. HSV-1 amplicon preparations using the 5dl1.2 helper virus was evaluated on Sodium currents and average spike frequency in response to depolarization. Transduction of cultured neurons with HSV-1 amplicon preparations using the 5dl1.2 helper virus selectively suppressed sodium currents by approximately 80% and reduced average spike frequency.
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