Factors Affecting the Growth of Pseudomonas fluorescens in Liquid Egg White

Pseudomonas fluorescens grew rapidly in fresh egg albumen diluted with water. Growth of the bacteria in egg albumen was stimulated by the addition of carbohydrate and ovomucoid-rich egg exudate. Polyacrylamide-gel electrophoresis for residual egg albumen revealed extensive proteolysis of albumen inoculated with the organism. A fluorescent compound with absorption maximum at 408 nm was isolated from a defined salt medium inoculated with P. fluorescens. It shortened the lag phase and increased the final cell yield of the organism when added to the salt medium (100 mug/ml).