What question did this study set out to answer?

To determine how maternal protein restriction during gestation affects β-cell gene expression and the DNA methylation status of Pdx1 and MafA regulatory regions in male rat offspring.

March 13, 2023Frontiers in Veterinary ScienceOpen Access

A low-protein maternal diet during gestation affects the expression of key pancreatic β-cell genes and the methylation status of the regulatory region of the MafA gene in the offspring of Wistar rats

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Authors

TSTonantzin C. Sosa-LariosInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán AOAna Laura Ortega-MárquezInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán JRJesús Rafael Rodríguez‐AguileraHelmholtz Centre for Environmental Research

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Implication

Animal study reveals gestational protein restriction alters islet gene expression and causes MafA hypomethylation in male rat offspring, indicating potential pathways to metabolic dysregulation.

Key Points

To determine how maternal protein restriction during gestation affects β-cell gene expression and the DNA methylation status of Pdx1 and MafA regulatory regions in male rat offspring.
Studied isolated pancreatic islets from male Wistar rat offspring born to dams fed either a protein-restricted or control diet during gestation.
Evaluated offspring at postnatal days 36 (juveniles) and 90 (young adults) for islet gene expression and DNA methylation patterns within the 5' flanking regulatory regions of Pdx1 and MafA.
At postnatal day 36, restricted offspring showed significant upregulation of Ins1, Ins2, Glut2, Pdx1, MafA, and Atf2, alongside downregulation of Gck and NeuroD1 compared with controls (P < 0.05).
DNA methylation levels decreased significantly in the 5' flanking region between nucleotides −8118 to −7750 of the MafA regulatory region in restricted offspring relative to controls.

Cite This Study

Sosa-Larios et al. (2023) studied this question.

synapsesocial.com/papers/6aa255afda8566d019e564eb https://doi.org/10.3389/fvets.2023.1138564

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Implication

Animal study reveals gestational protein restriction alters islet gene expression and causes MafA hypomethylation in male rat offspring, indicating potential pathways to metabolic dysregulation.

A low-protein maternal diet during gestation affects the expression of key pancreatic β-cell genes and the methylation status of the regulatory region of the MafA gene in the offspring of Wistar rats

Discussion

Member takes

Implication

Key Points

Cite This Study

A low-protein maternal diet during gestation affects the expression of key pancreatic β-cell genes and the methylation status of the regulatory region of the MafA gene in the offspring of Wistar rats

Discussion

Member takes

Implication

Key Points

Cite This Study

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