Evaluation of Antioxidant Activity Using by H2O2, DPPH, ABTS Methods and Phytochemical Tests, TPC & TFC of Commonly Used Medicinal Plants of West Bengal

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Medicinal plants are very useful in folk medicine due to present different phyto-components whose properties show medicinal importance. Antioxidant is one of them important properties which can protect cells from various types of cell damage by capturing free radicals. Various phyto-compounds are relatable for showing antioxidant activity of plants. The main aim of this study is that to investigate whether the relation between phenolic compound, flavonoid compound and antioxidant property is present or not. So, in this study was performed on twelve medicinal plant. Various phytochemical study is performed such as carbohydrate by Fehling’s, Benedict’s test, alkaloid test by dragendroff’s and Mayer’s method, protein by biuret and ninhydrin test, tannin by lead acetate, ferric chloride methods, and glycoside test. Besides phyto-chemicals test, total phenolic content (TPC), total flavonoid content (TFC) is performed by Folin Ciocalteau method, aluminium chloride colorimetric technique respectively. Antioxidant activity is measured by H2O2, DPPH, and ABTS method. Ascorbic acid and trolox are used as a standard of antioxidant test. Mandukparni and Bramhi show strong antioxidant property who’s TPC and TFC value falls in vicinity of highest range. According to the H2O2 method, IC50 value of Mandukparni is 50.06 mg/ml. Mandukparni show high antioxidant activity by using DPPH method, with IC50 value 50.22 mg/ml. In ABTS method, Bramhi showed highest antioxidant property with IC50 value 49.98mg/ml. There is a relation between TPC, TFC and antioxidant. But only the TPC, TFC is responsible for antioxidant that is not validate reasons. Various others phyto-compound synergistically or individually can influence the antioxidant properties.