Key result
Thromboxane A2 directly mobilizes Ca2+ without further phospholipase C activation in collagen-stimulated human platelets.
Thromboxane A2 directly causes calcium mobilization without further activation of phospholipase C during collagen-induced activation of human platelets.
Decouples thromboxane A2 from phospholipase C in platelet Ca2+ responses; hypothesis-generating for thrombosis mechanisms.
The thromboxane A2 antagonist, ONO-3708, completely inhibited the increase in cytosolic free Ca2+ in human platelets during activation with collagen. Half-maximal Ca2+ release and influx required about 3 and 4 nM STA2, a stable thromboxane A2 mimetic, respectively. However, half maximal activation of phospholipase C required about 18 nM STA2. This suggests that thromboxane A2 directly causes Ca2+ mobilization without further activation of phospholipase C during activation of human platelets with collagen.
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Moriyama et al. (1988) studied this question. ONO-3708 and STA2 was evaluated on Cytosolic free Ca2+ increase and phospholipase C activation. Thromboxane A2 directly causes Ca2+ mobilization without further activation of phospholipase C during activation of human platelets with collagen.
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