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September 10, 2026Turkish Journal of BiochemistryOpen Access

Downregulated miR-625-5p expression is linked to NAFLD and shows potential as a diagnostic biomarker.

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Why the study?

NAFLD is highly prevalent with complex pathogenesis, and the function and regulatory mechanism of miR-625-5p in NAFLD remained to be elucidated.

Does miR-625-5p overexpression reduce lipid accumulation, inflammatory response, and liver damage in NAFLD models?

Population

112 patients with NAFLD, 106 control subjects, and HepG2 and THLE-2 cells

Comparison

NAFLD vs control subjects and miR-625-5p overexpression vs controls in cell models

Design

Case-control and in vitro translational study

Key result

MiR-625-5p expression was significantly downregulated in patients with NAFLD compared to controls, suggesting its potential as a diagnostic biomarker.

Authors

MFMenghua FanSXShurui XieRZRongfang Zhai

Discussion

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Overview

Supports miR-625-5p as candidate NAFLD biomarker; hypothesis-generating for therapeutic targeting, requires prospective validation.

Key Points

  • To elucidate the clinical diagnostic value and downstream molecular regulatory mechanism of miR-625-5p in non-alcoholic fatty liver disease.
  • Enrolled 112 patients with NAFLD and 106 healthy control subjects, analyzing miR-625-5p diagnostic utility through ROC curve and logistic regression analyses.
  • Treated HepG2 and THLE-2 cell lines with oleic acid and palmitic acid to establish in vitro models of NAFLD.
  • Assessed triglyceride accumulation, AST/ALT enzymatic activity, inflammatory cytokines (IL-6, TNF-α), apoptosis, and direct CCND1 binding via RT-qPCR and dual-luciferase reporter assays.
  • MiR-625-5p expression was significantly downregulated in NAFLD patients, demonstrating significant diagnostic biomarker potential on ROC curve analysis.
  • Overexpression of miR-625-5p markedly reduced triglyceride levels, inflammatory factors, and markers of hepatocellular injury in fatty acid-treated cell models.
  • Dual-luciferase and correlation analyses confirmed that miR-625-5p directly binds and negatively regulates CCND1, whose upregulation reversed the therapeutic benefits of miR-625-5p.

Study Design

Type

Case-Control (n=218)

Structured PICO

Does miR-625-5p overexpression reduce lipid accumulation, inflammatory response, and liver damage in NAFLD models?

P
Population
218 participants, including 112 patients with NAFLD and 106 control subjects, evaluated for miR-625-5p expression.
E
Exposure
Overexpression of miR-625-5p (in vitro model)
C
Comparator
Control/NAFLD model without miR-625-5p overexpression
O
Outcome
Expression levels of miR-625-5p, diagnostic value (ROC), levels of triglyceride (TG), AST/ALT enzyme activity, apoptosis, and inflammatory factors (IL-6, TNF-α)surrogate

MiR-625-5p is downregulated in NAFLD and its overexpression impedes lipid accumulation and liver damage in vitro by targeting CCND1, suggesting potential as a biomarker and therapeutic target.

Cite This Study

Fan et al. (2026) conducted a case-control in Non-alcoholic fatty liver disease (NAFLD) (n=218). miR-625-5p expression vs. Control subjects was evaluated on Diagnostic value of miR-625-5p for NAFLD. MiR-625-5p expression was significantly downregulated in patients with NAFLD compared to controls, suggesting its potential as a diagnostic biomarker.

synapsesocial.com/papers/6aa27ad858559d80afc73b2chttps://doi.org/10.1515/tjb-2026-0101
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