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Stable hydrogen (δ 2 H) and oxygen (δ 18 O) isotopes in plant tissue water provide effective indicators for tracing fruit origin. This study optimized automatic cryogenic vacuum distillation (ACVD) system parameters (temperature, extraction time, and sample volume) to achieve a near‐complete water recovery and high analytical accuracy. Systematic deviations of isotopic values between the original water and the water extracted from the spiked strawberry samples (Δδ 2 H: 4.56‰; Δδ 18 O: 1.14‰) were observed, likely due to matrix effects during extraction. Nevertheless, the optimized ACVD protocol enabled clear discrimination of the geographical origins of strawberries, apples, and kiwifruits based on their δ 2 H and δ 18 O values. These results indicate that, despite extraction‐induced isotopic fractionation, tissue water isotopes remain reliable markers for fruit provenance when ACVD parameters are carefully controlled.
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He et al. (2025) studied this question.
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