Key result
Atrial natriuretic factor significantly elevated cGMP levels in rat aortic smooth muscle, an effect not prevented by suppression of the Na+-pump with ouabain or K+-free media.
Population
Freshly dissected rat aortic tissues (rat aortic smooth muscle)
Comparison
Atrial natriuretic factor 0.1 mumol/L in… vs Control media
Design
Preclinical
Authors
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ANF elevates rat aortic cGMP independently of Na+-pump activity; hypothesis-generating for ouabain-ANF interactions in human disease models.
The antagonism of ANF's vasorelaxant effects by ouabain is not mediated by preventing ANF-induced cGMP elevation in arterial smooth muscle.
Hegde et al. (1989) studied this question. Atrial natriuretic factor (r-ANF-8-33) vs. Control media, ouabain, and/or K+-free media was evaluated on cGMP levels in the aortic smooth muscle. Atrial natriuretic factor significantly elevated cGMP levels in rat aortic smooth muscle, an effect not prevented by suppression of the Na+-pump with ouabain or K+-free media.
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