What is the clinical evidence from this study?

Study design: Observational. Population: African swine fever virus (ASFV) infection (n=39). Intervention: ASFV genotype IX infection. Primary outcome: Genetic variation at four polymorphic loci (p72, p54, TK, B602L CVR).

November 15, 2017Virus GenesOpen Access

Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution

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Key result

Multi-locus sequence typing of 39 ASFV isolates revealed all clustered within p72 genotype IX, with rapid evolution and two previously unknown polymorphisms identified in the B602L central variable region.

Population

39 African swine fever virus isolates obtained from outbreaks in Kenya and a region of Eastern Uganda…

Authors

COCynthia OnzereWashington State University ABArmanda D. S. BastosUniversity of Pretoria EOEdward OkothKenya Medical Research Institute

Discussion

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Implication

Thymidine kinase locus variation offers a novel ASFV genotyping marker; leaves open its added value for surveillance pending validation.

Study Design

Type

Observational (n=39)

Structured PICO

Population

39 domestic pigs testing positive for African swine fever virus from outbreak and endemic regions of Kenya and Eastern Uganda between 2011 and 2013.

Exposure

Multi-locus sequence typing (p72, p54, thymidine kinase, and B602L central variable region)

Outcome

Genetic variation and phylogeographic evolution

Multi-locus sequence typing of ASFV isolates from East Africa reveals rapid evolution and dissemination of variants within genotype IX.

Limitations

Underestimation of viral prevalence when sampling blood alone due to tissue sequestration.
Anthropogenic factors such as rapid sale of pigs during outbreaks limit epidemiological accuracy.
Lack of direct evidence for sylvatic cycle vectors in the study area to explain the origin of novel variants.

Cite This Study

Onzere et al. (2017) conducted an observational in African swine fever virus (ASFV) infection (n=39). ASFV genotype IX infection was evaluated on Genetic variation at four polymorphic loci (p72, p54, TK, B602L CVR). Multi-locus sequence typing of 39 ASFV isolates revealed all clustered within p72 genotype IX, with rapid evolution and two previously unknown polymorphisms identified in the B602L central variable region.

synapsesocial.com/papers/6aa2a5bca841a277b27f6a64 https://doi.org/10.1007/s11262-017-1521-4

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Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

Also Consider

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