What question did this study set out to answer?

To directly compare the hematopoietic supportive capabilities of donor-matched umbilical cord-derived and placenta-derived mesenchymal stem cells as feeder layers for expanding umbilical cord blood CD34+ cells.

October 19, 2015Stem Cell Research & TherapyOpen Access

Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

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Authors

DKDarshana KadekarAarhus University VKVaijayanti KaleSymbiosis International University LLLalita LimayeUniversity of Mumbai

Discussion

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Implication

Comparative study demonstrates superior maintenance of primitive hematopoietic stem cells using placental versus cord mesenchymal feeders, indicating enhanced potential for cord blood expansion.

Key Points

To directly compare the hematopoietic supportive capabilities of donor-matched umbilical cord-derived and placenta-derived mesenchymal stem cells as feeder layers for expanding umbilical cord blood CD34+ cells.
Isolated CD34+ cells from umbilical cord blood and co-cultured them for 10 days on irradiated cord-derived (C-MSCs) or placenta-derived (P-MSCs) mesenchymal stem cells from the same donor.
Maintained cultures in serum-free medium supplemented with 25 ng/ml each of SCF, TPO, Flt-3 L, and IL-6.
Evaluated harvested cells for phenotype, colony-forming unit potential, long-term culture-initiating cell capacity, fibronectin adhesion, SDF-1α migration, and NOD/SCID mouse repopulation.
P-MSCs supported greater preservation of primitive CD34+CD38- cells, higher colony-forming unit output, increased long-term culture-initiating cell capacity, and superior NOD/SCID engraftment compared to C-MSCs.
P-MSCs mediated primitive stem cell maintenance via reduced apoptosis, enhanced Wnt and Notch signaling, HGF secretion, and direct cell contact, whereas C-MSCs favored committed progenitor differentiation via inflammatory cytokine release.

Cite This Study

Kadekar et al. (2015) studied this question.

synapsesocial.com/papers/6aa3133f1a2b81e9ac6745fe https://doi.org/10.1186/s13287-015-0194-y

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Implication

Comparative study demonstrates superior maintenance of primitive hematopoietic stem cells using placental versus cord mesenchymal feeders, indicating enhanced potential for cord blood expansion.

Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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