Bacteriological Profile and Antibiogram of Isolates from Pus Samples in a Tertiary Care Centre

Pus is one of the major samples received in the Microbiology laboratory for culture and sensitivity. Though the bacterial profile from pus samples remain similar in various studies, the inadvertent use of antibiotics has lead to the emergence of various drug resistant pathogens, which in turn acts as a great challenge to the health services. A total number of 233 pus samples obtained for aerobic culture and sensitivity from different IPDs and OPDs of Owaisi Group of Hospitals associated with Deccan College of Medical Sciences, Hyderabad during a period from June 2017 to October 2017 were processed using standard microbiological processed and antibiotic susceptibility testing was done as per CLSI guidelines. Analysis of 233 pus samples showed 65.2% culture positivity (M:F=1.30:1.00) with Surgical wards (33.5%) being the major contributor. Klebsiella spp (36.2%) was the most common organism followed by Staphylococcus aureus (21.7%). Antibiotic sensitivity profile of Gram negative bacteria showed sensitivity towards imipenem (92.7%), piperacillin (89.1%) and amikacin (87.2%). Staphylococcus aureus was susceptible to Linezolid (100%), Vancomycin (100%). The prevalence of MRSA was 12.12%. A continuous inspection should be carried out to monitor the antimicrobial susceptibility of bacterial isolates from pus samples to chose appropriate antibiotics for prophylaxis and treatment of infections.