Key result
Inhibiting S-acylation attenuates endogenous BK channel currents by disrupting functional coupling to regulatory β1-subunits.
Why the study?
Mechanisms that control the functional coupling of BK channel pore-forming alpha-subunits with regulatory subunits are poorly understood.
S-acylation dynamically regulates the functional coupling of BK channel α-subunits with β1-subunits, providing a mechanism for cell-specific control of ion-channel physiology.
Hypothesis-generating for BK channel modulation in vascular models; leaves open human therapeutic relevance.
The properties and physiological function of pore-forming α-subunits of large conductance calcium- and voltage-activated potassium (BK) channels are potently modified by their functional coupling with regulatory subunits in many tissues. However, mechanisms that might control functional coupling are very poorly understood. Here we show that S -acylation, a dynamic post-translational lipid modification of proteins, of the intracellular S0–S1 loop of the BK channel pore-forming α-subunit controls functional coupling to regulatory β1-subunits. In HEK293 cells, α-subunits that cannot be S -acylated show attenuated cell surface expression, but expression was restored by co-expression with the β1-subunit. However, we also found that nonacylation of the S0–S1 loop reduces functional coupling between α- and β1-subunits by attenuating the β1-subunit-induced left shift in the voltage for half-maximal activation. In mouse vascular smooth muscle cells expressing both α- and β1-subunits, BK channel α-subunits were endogenously S -acylated. We further noted that S -acylation is significantly reduced in mice with a genetic deletion of the palmitoyl acyltransferase (Zdhhc23) that controls S -acylation of the S0–S1 loop. Genetic deletion of Zdhhc23 or broad-spectrum pharmacological inhibition of S -acylation attenuated endogenous BK channel currents independently of changes in cell surface expression of the α-subunit. We conclude that functional effects of S -acylation on BK channels depend on the presence of β1-subunits. In the absence of β1-subunits, S -acylation promotes cell surface expression, whereas in its presence, S -acylation controls functional coupling. S -Acylation thus provides a mechanism that dynamically regulates the functional coupling with β1-subunits, enabling an additional level of conditional, cell-specific control of ion-channel physiology.
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Duncan et al. (2019) studied this question. S-acylation modification / Zdhhc23 deletion vs. Wild-type / uninhibited state was evaluated on Functional coupling between α- and β1-subunits and BK channel currents. S-acylation of the BK channel pore-forming α-subunit dynamically controls its functional coupling to regulatory β1-subunits, and its inhibition attenuates endogenous BK channel currents.
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