What is the clinical evidence from this study?

Population: Lipid metabolism (animal model). Intervention: Chylomicrons and their remnants vs. VLDL and their remnants. Primary outcome: Clearance half-life (t1/2).

What question did this study set out to answer?

To determine whether the hepatic binding and uptake mechanisms for triglyceride-rich lipoprotein remnants differ based on whether they originate from the intestine or the liver.

November 1, 1982AJP Gastrointestinal and Liver Physiology

Comparison of binding and removal of remnants of triglyceride-rich lipoproteins of intestinal and hepatic origin by rat liver in vitro

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Key result

Chylomicrons were cleared more quickly than VLDL (t1/2 3.7 vs 10.6 min), but their remnants shared the same hepatic removal mechanism and displaced each other with equal potency.

Key Points

To determine whether the hepatic binding and uptake mechanisms for triglyceride-rich lipoprotein remnants differ based on whether they originate from the intestine or the liver.
Isolated chylomicrons from rat intestinal lymph and very low-density lipoproteins (VLDL) from isolated perfused rat livers, then generated remnants in eviscerated rats.
Measured in vivo clearance rates and in vitro remnant uptake using isolated perfused rat livers challenged with 125I-labeled remnants alongside unlabeled competitors.
Assessed specific binding and cross-displacement of radiolabeled chylomicron and VLDL remnants in isolated rat liver plasma membrane preparations.
In vivo initial clearance was more rapid for nascent chylomicrons than nascent VLDL (half-life of 3.7 ± 1.4 min vs. 10.6 ± 4.0 min), while VLDL remnants were cleared more rapidly than nascent VLDL.
A 15- to 20-fold excess of either unlabeled chylomicron remnants or VLDL remnants inhibited roughly 50% of the hepatic uptake of 125I-labeled remnants, showing equivalent competitive potency.
Both chylomicron and VLDL remnants bound specifically to isolated liver plasma membranes and displaced each other with equal efficiency.

Structured PICO

Population

In vivo and in vitro study using rat models to evaluate the clearance and hepatic binding of chylomicron and VLDL remnants.

Intervention

Chylomicron remnants and VLDL remnants

Comparator

Nascent VLDL; cross-competition between remnant types

Outcome

Hepatic removal rate and binding to liver plasma membranessurrogate

Main Result

Absolute Event Rate: 3.7% vs 10.6%

Chylomicron and VLDL remnants share the same hepatic removal mechanism, suggesting that the rate of removal is not dependent on the organ of origin of the precursor lipoprotein.

Discussion

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Implication

Animal data indicate shared hepatic remnant removal; leaves open whether intestinal and hepatic lipoproteins compete in human disease.

Cite This Study

Cooper et al. (1982) studied Lipid metabolism (animal model). Chylomicrons and their remnants vs. VLDL and their remnants was evaluated on Clearance half-life (t1/2). Chylomicrons were cleared more quickly than VLDL (t1/2 3.7 vs 10.6 min), but their remnants shared the same hepatic removal mechanism and displaced each other with equal potency.

synapsesocial.com/papers/6aa35efe407158ff5cc7ef0b https://doi.org/10.1152/ajpgi.1982.243.5.g389

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Comparison of binding and removal of remnants of triglyceride-rich lipoproteins of intestinal and hepatic origin by rat liver in vitro

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Comparison of binding and removal of remnants of triglyceride-rich lipoproteins of intestinal and hepatic origin by rat liver in vitro

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Also Consider

Also Consider