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Flavanones reduced with potassium borohydride and acidified with hydrochloric acid develop red to violet colors. This reaction forms the basis for a quantitative spectrophotomerric method for the determination of flavanones. The procedure outlined is sufficiently sensitive to determine quantitatively solubilized flavanones at concentrations of 0.02 mg./ml. Determinations of flavanone content of typical pharmaceutical formulations containing citrus bioflavonoids demonstrate complete recovery of added bioflavonoids. The method is rapid and the results are reproducible.
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Rowell et al. (1959) studied this question.
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