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Review the development and application of genetic approaches in Saccharomyces cerevisiae to dissect the molecular mechanisms and factor requirements of eukaryotic translation initiation.

January 1, 2000Cold Spring Harbor Monograph Archive

12 Genetic Approaches to Translation Initiation in Saccharomyces cerevisiae

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Authors

TDThomas F. DonahueKansas State University

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Implication

Narrative review demonstrates the power of yeast genetics for dissecting translation initiation, highlighting how suppressor analysis identifies novel initiation factors.

Key Points

Review the development and application of genetic approaches in Saccharomyces cerevisiae to dissect the molecular mechanisms and factor requirements of eukaryotic translation initiation.
Examined genetic suppressor analysis targeting genes encoding eukaryotic translation initiation factors in Saccharomyces cerevisiae.
Integrated yeast genetic strategies with classical biochemical isolation techniques developed in mammalian and yeast model systems.
Genetic suppressor analysis effectively resolves the functions of individual subunits within multisubunit initiation factors that resist standard biochemical characterization.
Demonstrated that combining genetic screens with biochemistry provides a highly sensitive method to detect previously uncharacterized translation initiation factors and define discrete pathway steps.

Cite This Study

Thomas F. Donahue (2000) studied this question.

synapsesocial.com/papers/6aa3830834a74eb94ec7f2f2 https://doi.org/10.1101/087969618.39.487

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Implication

Narrative review demonstrates the power of yeast genetics for dissecting translation initiation, highlighting how suppressor analysis identifies novel initiation factors.

12 Genetic Approaches to Translation Initiation in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

12 Genetic Approaches to Translation Initiation in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

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