April 4, 2003Proceedings of the National Academy of Sciences

Isolation and identification of short nucleotide sequences that affect translation initiation in Saccharomyces cerevisiae

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Population

Saccharomyces cerevisiae (yeast strain with endogenous HIS3 gene deleted)

Comparison

Library of constructs expressing dicistronic… vs Untransformed cells or those containing the…

Design

Preclinical

Authors

WZWei ZhouChongqing University GEGerald M. EdelmanRockefeller Foundation VMVincent P. MauroScripps Research Institute

Discussion

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Implication

Short synthetic IRESes function in yeast; leaves open any extension to mammalian cardiovascular gene therapy.

Structured PICO

Population

Saccharomyces cerevisiae (yeast strain with endogenous HIS3 gene deleted)

Intervention

Library of constructs expressing dicistronic mRNAs with the HIS3 gene as the second cistron and 18 random nucleotides in the intercistronic region

Comparator

Untransformed cells or those containing the parent construct

Outcome

Growth on medium lacking histidine (indicating HIS3 expression)surrogate

The identification of short nucleotide sequences that facilitate internal translation initiation in yeast enables detailed biochemical analyses and potential bioengineering applications.

Cite This Study

Zhou et al. (2003) studied this question.

synapsesocial.com/papers/6a7c1139a540d02696c2bf96 https://doi.org/10.1073/pnas.0437993100

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Isolation and identification of short nucleotide sequences that affect translation initiation in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Isolation and identification of short nucleotide sequences that affect translation initiation in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider