Key result
Yeast cell extracts can initiate cap-independent translation of mRNAs containing viral and cellular internal ribosome entry sites (IRES), demonstrating that an internal translation initiation mechanism exists in yeast.
Population
Cell extracts prepared from various strains of Saccharomyces cerevisiae
Comparison
Viral and cellular 5' noncoding regions vs Other translation systems and cap-dependent…
Design
Preclinical
Authors
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Internal initiation occurs in yeast extracts; extends IRES mechanisms beyond mammals and leaves open in vivo roles.
Demonstrates that yeast cell extracts can initiate cap-independent translation via an internal ribosome binding mechanism, mimicking in vivo effects.
Iizuka et al. (1994) studied this question. In vitro translation using yeast cell extracts was evaluated on Translation efficiency of various mRNA constructs. Yeast cell extracts can initiate cap-independent translation of mRNAs containing viral and cellular internal ribosome entry sites (IRES), demonstrating that an internal translation initiation mechanism exists in yeast.
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