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November 1, 1994Molecular and Cellular Biology

Cap-Dependent and Cap-Independent Translation by Internal Initiation of mRNAs in Cell Extracts Prepared from Saccharomyces cerevisiae

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Key result

The 5' noncoding regions of hepatitis C virus, coxsackievirus B1, and yeast mRNAs encoding TFIID and HAP4 mediated cap-independent translation in yeast cell extracts.

Population

Cell extracts prepared from various strains of Saccharomyces cerevisiae

Comparison

5'-terminal cap structures, 3'-terminal poly… vs Other translation systems and cap-dependent…

Design

Preclinical

Authors

NINarushi IizukaNagoya City UniversityLNLyle NajitaUniversity of Colorado HealthAFAlex FranzusoffNational Institutes of Health

Discussion

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Implication

No immediate clinical implications; leaves open whether IRES mechanisms operate in mammalian cells or human disease.

Structured PICO

P
Population
Cell extracts prepared from various strains of Saccharomyces cerevisiae
I
Intervention
5'-terminal cap structures, 3'-terminal poly(A) sequences, and 5' noncoding regions of viral (HCV, coxsackievirus B1) and cellular (TFIID, HAP4) mRNAs
C
Comparator
Other translation systems (rabbit reticulocytes, wheat germ, human HeLa cells) and cap-dependent translation
O
Outcome
Translation efficiency and capability of initiating cap-independent translationsurrogate

Yeast translation extracts reveal that specific viral and cellular mRNA leader sequences can initiate translation via an internal ribosome binding mechanism, suggesting a regulatory mechanism for endogenous mRNAs.

Cite This Study

Iizuka et al. (1994) studied this question. In vitro translation in yeast extracts was evaluated. The 5' noncoding regions of hepatitis C virus, coxsackievirus B1, and yeast mRNAs encoding TFIID and HAP4 mediated cap-independent translation in yeast cell extracts.

synapsesocial.com/papers/6a92d08e87bb163ace623390https://doi.org/10.1128/mcb.14.11.7322-7330.1994
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism1993 · 444 citations
  2. 2Internal ribosome entry site within hepatitis C virus RNA1992 · 925 citations
  3. 3Functional analysis of the internal translation initiation site of foot-and-mouth disease virus1990 · 203 citations
  4. 4Nucleotide sequences important for translation initiation of enterovirus RNA1991 · 52 citations
  5. 5The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro1991 · 211 citations