Key result
The addition of a methylated cap or a poly(A) tail enhanced translation and stabilized mRNA in yeast cell-free extracts, with capping dependence reduced by the HSP26 leader sequence.
This basic science study demonstrates that 5'-capping and 3'-polyadenylation enhance mRNA translation and stability in yeast cell-free extracts, with specific leader sequences potentially enabling cap-independent translation.
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Does not support changes to mRNA therapeutics; leaves open whether HSP26-like sequences enable cap-independent translation in mammalian systems.
Gerstel et al. (1992) studied this question. 5'-capping, 3'-polyadenylation and leader sequence vs. uncapped/unpolyadenylated mRNA was evaluated on translation and stability of mRNA. The addition of a methylated cap or a poly(A) tail enhanced translation and stabilized mRNA in yeast cell-free extracts, with capping dependence reduced by the HSP26 leader sequence.
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