What question did this study set out to answer?

To determine whether vaccination with an inactivated molecularly cloned virus or its derived recombinant envelope and gag proteins protects macaques from a virulent SIV challenge.

February 1, 1992Journal of Medical Primatology

Protection against SIV infection in macaques by immunization with inactivated virus from the BK28 molecular clone, but not with BK28‐derived recombinant env and gag proteins

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Authors

KMKingston H. G. MillsUniversity College Dublin MPMark PageEnvironment Agency WCWoon Ling ChanNational Yang Ming Chiao Tung University

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Implication

Animal experimental study demonstrates protection against SIV challenge via inactivated cloned virus rather than recombinant proteins in macaques, highlighting the role of anti-cell antibodies.

Key Points

To determine whether vaccination with an inactivated molecularly cloned virus or its derived recombinant envelope and gag proteins protects macaques from a virulent SIV challenge.
Immunized cynomolgus macaques with either beta-propiolactone-inactivated SIVmacBK28 in Freund's adjuvant or purified recombinant SIV env and gag proteins in Syntex adjuvant.
Challenged vaccinated animals with the heterogeneous 32H isolate of SIVmac251 propagated in C8166 cells.
Measured cellular and humoral immune responses, including anti-envelope antibodies, T-cell activity, and anti-cell antibodies.
Inactivated SIVmacBK28 induced low but detectable anti-env antibodies and T-cell responses alongside high anti-cell antibodies, conferring protection against infection following challenge with the 32H isolate.
Recombinant BK28-derived env and gag proteins generated consistent and long-lived cellular and humoral anti-env responses but failed to protect macaques against the 32H viral challenge.

Cite This Study

Mills et al. (1992) studied this question.

synapsesocial.com/papers/6aa397c634a74eb94ec7f582 https://doi.org/10.1111/j.1600-0684.1992.tb00567.x

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Implication

Animal experimental study demonstrates protection against SIV challenge via inactivated cloned virus rather than recombinant proteins in macaques, highlighting the role of anti-cell antibodies.

Protection against SIV infection in macaques by immunization with inactivated virus from the BK28 molecular clone, but not with BK28‐derived recombinant env and gag proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

Protection against SIV infection in macaques by immunization with inactivated virus from the BK28 molecular clone, but not with BK28‐derived recombinant env and gag proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

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