Key result
Segmental isotopic labeling reveals independent folding of a 64-nucleotide domain within the intact HCV IRES.
The study presents a method for segmental isotopic labeling of RNA fragments, demonstrating its utility in analyzing the structure of large biological RNAs like the HCV IRES.
Answers are grounded in this paper and cite their sources.
Enables structural dissection of large viral RNAs; extends isotopic labeling methods but leaves open therapeutic applications.
RNA NMR is hindered by the large size of most biological RNAs. We present here a simple method for segmental isotopic labeling of an RNA fragment within the context of a larger RNA. The methodology uses transcription and ribozyme cleavage to prepare appropriate ends for RNA ligase catalyzed ligation. We demonstrate that a 64 nucleotide domain of the Hepatitis C virus internal ribosome entry site (IRES) RNA adopts an independently folded domain within the context of the intact, 100 kDa IRES.
No takes yet. Share an insight, caveat, or question.
Kim et al. (2002) studied this question. Segmental isotopic labeling was evaluated on Independent folding of a 64 nucleotide domain within the 100 kDa IRES. Segmental isotopic labeling demonstrated that a 64 nucleotide domain of the Hepatitis C virus IRES RNA adopts an independently folded domain within the intact 100 kDa IRES.
Synapse has enriched 10 related papers. Consider them for comparative context: