Key result
Metal-based fluorescent sensors detect nitric oxide and reveal NO disassembles Fe-S clusters.
Metal-based fluorescent sensors can detect NO in living cells, and NO disassembles Fe-S clusters to form dinitrosyl iron complexes.
May enable cellular NO imaging in cardiovascular models; leaves open relevance to human disease and requires validation before any therapeutic consideration.
We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron-sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron-sulfur proteins. Our studies reveal that NO disassembles the Fe-S clusters to form dinitrosyl iron complexes.
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Tonzetich et al. (2010) studied this question. Metal-based fluorescent sensors and synthetic model complexes of iron-sulfur clusters was evaluated on Detection of NO and reactivity of iron-sulfur clusters toward NO. Metal-based fluorescent sensors permitted identification of NO from nitric oxide synthase, and synthetic model complexes revealed that NO disassembles Fe-S clusters to form dinitrosyl iron complexes.
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