What question did this study set out to answer?

Review the biological roles, pharmacology, and toxicology of nitric oxide while evaluating existing techniques for measuring its production in biological systems.

February 1, 1993The FASEB Journal

Measurement of nitric oxide in biological models

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Authors

SAStephen L. ArcherGeneral Cardiology

Discussion

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Implication

Review evaluates detection methods for nitric oxide in biological models, highlighting chemiluminescence and microelectrodes as sensitive analytical tools.

Key Points

Review the biological roles, pharmacology, and toxicology of nitric oxide while evaluating existing techniques for measuring its production in biological systems.
Conducted a computerized literature review spanning five years of research addressing endothelium-derived relaxing factor (EDRF).
Assessed standard detection strategies including spin-trapping adducts, methemoglobin oxidation, and ozone-induced chemiluminescence alongside novel electrochemical microelectrodes.
Identified direct nitric oxide quantification in fewer than 50 of 955 published articles on EDRF.
Documented detection thresholds of approximately 1 nmol for electron paramagnetic resonance (EPR) and spectrophotometry, compared to approximately 20 pmol for ozone chemiluminescence.
Identified amperometric microelectrode assays with detection limits reaching 10⁻²⁰ M, enabling localized measurements in intact tissue and single cells.

Cite This Study

Stephen L. Archer (1993) studied this question.

synapsesocial.com/papers/6a15cd5c37103a4337a034a3 https://doi.org/10.1096/fasebj.7.2.8440411

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February 1, 1993The FASEB Journal

Measurement of nitric oxide in biological models

View Full Paper

Ask AI

Bookmark

Authors

SAStephen L. ArcherGeneral Cardiology

Discussion

Member takes

Implication

Review evaluates detection methods for nitric oxide in biological models, highlighting chemiluminescence and microelectrodes as sensitive analytical tools.

Key Points

Review the biological roles, pharmacology, and toxicology of nitric oxide while evaluating existing techniques for measuring its production in biological systems.
Conducted a computerized literature review spanning five years of research addressing endothelium-derived relaxing factor (EDRF).
Assessed standard detection strategies including spin-trapping adducts, methemoglobin oxidation, and ozone-induced chemiluminescence alongside novel electrochemical microelectrodes.
Identified direct nitric oxide quantification in fewer than 50 of 955 published articles on EDRF.
Documented detection thresholds of approximately 1 nmol for electron paramagnetic resonance (EPR) and spectrophotometry, compared to approximately 20 pmol for ozone chemiluminescence.
Identified amperometric microelectrode assays with detection limits reaching 10⁻²⁰ M, enabling localized measurements in intact tissue and single cells.

Cite This Study

Stephen L. Archer (1993) studied this question.

synapsesocial.com/papers/6a15cd5c37103a4337a034a3 https://doi.org/10.1096/fasebj.7.2.8440411

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