Key result
Pretreatment of human serum with 5-sulfosalicylic acid produced deglycosylated congeners of digoxin, decreasing measured digoxin to 50% in the ACS assay while causing little change in TDx and RIA.
Population
Human serum samples from 5 patients and normal plasma containing digoxin and endogenous digoxin-like…
Design
Other
Authors
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May artifactually lower ACS digoxin readings; leaves open assay standardization needs for reliable TDM.
Breakdown of digoxin and DLIF during serum pretreatment with SSA can compromise the accuracy of specific immunoassays, highlighting the need to understand pretreatment effects on assay reliability.
Qazzaz et al. (1995) studied this question. 5-sulfosalicylic acid (SSA) pretreatment was evaluated on Measured digoxin levels and breakdown products. Pretreatment of human serum with 5-sulfosalicylic acid produced deglycosylated congeners of digoxin, decreasing measured digoxin to 50% in the ACS assay while causing little change in TDx and RIA.
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