Key result
Elevated potassium and reduced temperature slow conduction velocity up to ~35% in perfused rat hearts.
Population
Langendorff-perfused rat hearts
Comparison
Voltage-sensitive dye mapping using… vs Physiological conditions and non-immobilized…
Design
Preclinical
Authors
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Should not yet guide management of hyperkalemia or hypothermia; leaves open translation to human arrhythmogenesis.
Voltage-sensitive dye mapping with di-4-ANEPPS in Langendorff-perfused rat hearts allows for detailed characterization of conduction velocity and activation patterns, though the dye itself can cause PQ interval prolongation and AV block.
Nygren et al. (2003) studied this question. Hyperkalemia and hypothermia vs. Physiological conditions was evaluated on Conduction velocity. Elevation of extracellular potassium to 9 mM and reduction of temperature to 32°C slowed conduction velocity in Langendorff-perfused rat hearts by 35% and 28%, respectively.
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