Key result
Two murine hybridoma lines produce monoclonal antibodies highly specific for cardiac troponin-I.
The successful production of highly specific anti-cardiac troponin-I monoclonal antibodies provides a potential tool for future in vivo imaging of myocardial infarction and myocyte damage.
Cardiac troponin-I (cTn-I) was isolated from bovine left ventricular tissue and used as immunogen. Sixteen murine hybridoma lines were produced with two of them, 1D12 and 5F4, showing a high specificity for cTn-I; both of these monoclonal antibodies (McAbs) were isotyped as IgG1 with kappa-light chains. The specificity of the McAbs for cTn-I was confirmed by ELISA, western blotting and by the ability of the antibodies to block actomyosin ATPase inhibition by cTn-I. The McAbs may be useful for human in vivo imaging of myocardial infarcts and other pathological conditions related to cardiac myocyte damage.
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Haider et al. (1994) studied this question. Anti-cardiac troponin-I monoclonal antibodies (1D12 and 5F4) was evaluated on Specificity for cardiac troponin-I. Two murine hybridoma lines, 1D12 and 5F4, produced monoclonal antibodies with high specificity for cardiac troponin-I, confirmed by ELISA, western blotting, and ATPase inhibition blocking.
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