Experimental study demonstrates altered neutrophil marker expression in healthy individuals, highlighting the risk of misinterpreting laboratory immunophenotyping.
The interest of developing functional and other experimental procedures on polymorphonuclear neutrophils (PMN) for the study of inflammatory disorders and primary immunodeficiencies is constantly increased.However, these experimental manipulations may induce significant changes on neutrophils' immunophenotyping and function.For this purpose, we investigated the effect of different experimental procedures (such as resting state, isolation and incubation with and without inflammatory stimuli) on the immunophenotype of PMN of healthy individuals, evaluating the expression pattern of specific granulocytic markers, important for chemotaxis, adhesion, opsonization, migration and phagocytosis.Our results indicate that the effect of experimental manipulation on PMN should always be taken into consideration in order to avoid misinterpreting the experimental findings.
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Tsinti et al. (2018) studied this question.
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