Key result
Multiplexed liquid array assay shows strong recombinant-peptide correlation for detecting NSP antibodies in cattle.
A multiplexed liquid array assay can effectively detect FMD virus nonstructural protein antibodies, offering a high-throughput method to differentiate infected from vaccinated animals.
May support multiplexed DIVA testing in FMD cattle; leaves open field validation and broader species application.
Liquid array technology was used to develop a multiplexed assay for the detection of antibodies to viral nonstructural proteins (NSPs), raised in cattle in response to infection with foot-and-mouth disease (FMD) virus. Two assays, one based on recombinant NSPs and the other on synthetically produced peptides, were developed and compared side-by-side. Serum samples from serial bleeds of cattle, each experimentally infected with one of the seven serotypes (C, A, O, Asia, SAT1, SAT2, SAT3) of FMD virus were analyzed. A distinct pattern in the detection of NSP antibodies and a close correlation of the recombinant protein and peptide-based assays were observed. The detection of antibodies to NSPs is a method to differentiate FMD-infected and FMD-vaccinated animals, and a high-throughput assay would be an invaluable tool in the case of an outbreak of FMD in North America, when emergency vaccination may be utilized to spare vaccinated, noninfected animals from slaughter and subsequent disposal.
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Perkins et al. (2006) studied Foot-and-mouth disease (FMD) virus infection. Multiplexed assay based on recombinant NSPs vs synthetically produced peptides was evaluated on Detection of NSP antibodies. A multiplexed liquid array assay demonstrated a distinct pattern in detecting NSP antibodies in FMD-infected cattle, with a close correlation between recombinant protein and peptide-based assays.
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