Key result
Sequencing of FMDV vaccine candidate O/YM/YN/2000 reveals an 8,133 nucleotide genome with specific deletion mutations.
Genomic data on FMDV strain informs vaccine candidate selection; leaves open immunogenicity and field efficacy.
The complete genome of foot-and-mouth disease virus (FMDV) vaccine candidate strain O/YM/YN/2000 was sequenced and analyzed. Seven overlapping cDNA fragment which covered the O/YM/YN/2000 genome were amplified by RT-PCR and sequenced. The results showed that the complete genome of the strain O/YM/YN/2000 has 8 133 nucleotides (nt) in length including a 1 053 nt 5′- untranslated region (UTR) (including 18 nt poly C), a 6 969 nt open reading frame (ORF), a 93 nt 3′-UTR and at least 18 nt poly A tail. The homologous comparison and phylogenetic analysis of the nucleotide and deduced amino acid (aa) sequences among O/YM/YN/2000 and other FMDV strains available deposited in GenBank were performed. Phylogenetic analysis based on the complete genome and VP1 revealed that that the strain O/YM/YN/2000 is clustered in the FMDV serotype O cathay topotype. Fragment deletions mutation in O/YM/YN/2000 were revealed, deletions involving 4 nt in the S fragment of 5′-UTR, 43 nt which possibly contributed to the loss of pseudo-knot domain II of 5′-UTR and 10 aa residues in 3A protein. The results in this research might provide some information for screening FMDV vaccine strain
No takes yet. Share an insight, caveat, or question.
Li Huachun (2011) studied Foot-and-mouth disease virus (FMDV). Whole genome sequencing of FMDV vaccine candidate strain O/YM/YN/2000 was evaluated on Complete genome sequence and phylogenetic analysis. Whole genome sequencing of FMDV vaccine candidate strain O/YM/YN/2000 revealed an 8,133 nucleotide genome clustered in the serotype O cathay topotype with specific fragment deletion mutations.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: