Vasodilator-stimulated phosphoprotein (VASP) is not a major mediator of platelet aggregation, thrombogenesis, haemostasis, and antiplatelet effect of prasugrel in rats

Figures

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  Figure 1. Vasodilator-stimulated phosphoprotein (VASP) expression in VASP+/+, VASP+/−, and VASP−/− rats. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis were performed with 10 μg of protein extracted from each organ. The primary antibody used for VASP was VASP antibody (3112 S, Cell Signaling Technology, Inc.) with 1,000-fold dilution. n = 3 per genotype. +/+, wild type; +/−, heterozygous VASP knockout; −/−, homozygous VASP knockout.

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  Figure 2. Impact of vasodilator-stimulated phosphoprotein (VASP) deficiency on collagen-induced thrombocytopenia in rats. Closed bars: VASP+/+; dotted bars: VASP+/−; open bars: VASP−/−. Data are means ± SEM (n = 4 or 5). ***P < 0.0001 vs. vehicle (Dunnett’s multiple comparison test). †P < 0.05 vs. VASP+/+ (Student’s t-test). AUC, area under the curve.

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  Figure 3. Effect of vasodilator-stimulated phosphoprotein (VASP) knockout on thrombus formation in FeCl3-induced arterial thrombosis in rats. Typical tracings of blood flow, haematoxylin and eosin (HE), and Elastica van Gieson (EVG) are presented for each genotype. Summary statistics of time in seconds to first occlusion (TTO), patency rate, vessel area, thrombus area, and stenosis ratio are also presented. Data are means ± SEM (n = 10).

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  Figure 4. Influence of vasodilator-stimulated phosphoprotein (VASP) knockout on phosphorylated VASP antigen, platelet reactivity index (PRI), and inhibition of platelet aggregation (IPA) in rats. (A) Optical density at 450 nm reflecting quantity of phosphorylated VASP antigen in VASP+/+, VASP+/−, and VASP−/− rats. Data are means ± SEM (n = 5). ***P < 0.0001 vs. VASP+/+. (B) Effect of R-138727 on platelet reactivity index in VASP+/+ and VASP+/− rats. Data are means ± SEM (n = 5). ***P < 0.0001 vs. vehicle in VASP+/+ rats. †P < 0.05, †††P < 0.0001 vs. vehicle in VASP+/− rats. (C,D) Effect of R-138727 on ADP- or collagen-induced platelet aggregation in VASP+/+ and VASP−/− rats. Data are means ± SEM (n = 5). *P < 0.05, ***P < 0.0001 vs. vehicle in VASP+/+ rats. †P < 0.05, †††P < 0.0001 vs. vehicle in VASP−/− rats.