Differential Expression of SERCA and Myosin Heavy Chain Isoforms in Rat Laryngeal Muscles

Figures

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  Fig. 1. Effect of aging on SERCA and MHC isoform expression in intrinsic laryngeal muscles. C Charts show the percentages of SERCA1-, SERCA2-, and co-expression-positive fibers in young and aged rats in the posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), superior cricoarytenoid (SCA), and cricothyroid (CT).

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  Fig. 2. Immunohistochemical detection of MHCI and MHCII expression in intrinsic laryngeal muscle fibers reveals spatially distinct patterns. (A-C) MHCII (fast fiber) expression in young rats was immunohistochemically evaluated in (A) the Posterior Cricoarytenoid (PCA), (B) the thyroarytenoid (TA), and (C) the cricothyroid (CT) muscles. MHCII fast fibers were predominantly expressed by TA, but not by CT. Scale bars = 100 µm (×200). (D–F) MHCI (slow fiber) expression in young rats was immunohistochemically evaluated in (D) the posterior cricoarytenoid (PCA), (E) the thyroarytenoid (TA), and (F) the cricothyroid (CT). MHCI slow fibers were highly expressed in the CT, whereas the PCA and TA showed lower expression. Scale bars = 100 µm (x 200).

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  Fig. 3. Effect of denervation on SERCA and MHC isoform expression in affected and non-affected contralateral PCA muscle.

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  Fig. 4. Effect of denervation on SERCA and MHC isoform expression in affected and non-affected contralateral PCA muscle after 2 weeks. Expression of MHCII (green) and SERCA2 (red) in PCA was evaluated in 2weeks after denervation. Micrographs depict the non-treated contralateral side (A-C) and the denervated side (D-F). Scale bars = 100 µm (×200).

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  Fig. 5. Effect of denervation on muscle fiber expression in PCA. Expression of SERCA1 (green) and SERCA2 (red) in PCA was evaluated 12 weeks after denervation. Micrographs depict the non-treated contralateral side (A-C) and the denervated side (D-F). Scale bars = 100 µm (×200).

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  Fig. 6. Effect of denervation on SERCA and MHC isoform expression in CT muscle.

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  Fig. 7. Effect of denervation on SERCA and MHC isoform expression in CT muscle, demonstrated by immunohistochemistry. Expression of SERCA1 (green) and SERCA2 (red) in CT was evaluated 2 weeks after denervation. Micrographs depict the non-treated contralateral side (A-C) and the denervated side (D-F). Scale bars = 100 µm (×100).