Key result
FMP dye maximizes signal-to-background ratio and FRET dyes respond fastest in ion channel assays.
Population
RBL-2H3 cells expressing an endogenous inward rectifier potassium channel (IRK1)
Comparison
4 membrane potential probes: oxonol dye DiBAC… vs Comparison among the 4 probes
Design
Preclinical
Authors
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FMP and PanVera FRET dyes may aid ion channel assay optimization; leaves open translation to cardiovascular applications.
The FMP dye and PanVera FRET dyes offer distinct advantages for high-throughput screening of ion channels, though they are unable to measure gramicidin-induced cell membrane hyperpolarizations.
Wolff et al. (2003) studied Ion channel screening. Membrane potential-sensitive fluorescent probes was evaluated on Signal over background ratio, response speed, and IC50 correlation. The FMP dye presented the highest signal-to-background ratio and PanVera FRET dyes gave the fastest response in ion channel screening assays.
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