Key result
FLIPR assay kit detects membrane potential changes faster and more sensitively than DiBAC4.
Population
In vitro model for evaluating potassium channel (ATP-sensitive K+ channel; K(ATP)) modulators
Comparison
FLIPR Membrane Potential Assay Kit (FMP) vs DiBAC4(3)-based FLIPR assays
Design
Preclinical
Authors
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May streamline preclinical HTS of KATP modulators; extends DiBAC4(3) methods but leaves open clinical translation.
The FMP dye provides a more sensitive and rapid alternative to DiBAC4(3) for high throughput screening of potassium channel modulators.
Whiteaker et al. (2001) studied In vitro assay for potassium channel modulators. FLIPR Membrane Potential Assay Kit (FMP) vs. DiBAC4(3) was evaluated on Changes in membrane potential and response time. The FLIPR Membrane Potential Assay Kit provided a more sensitive evaluation of changes in membrane potential with a more rapid response time relative to DiBAC4(3).
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