Key result
Novel MPSD assay for HCN modulators shows low variability and moderate correlation with standard electrophysiology.
Population
Cells expressing HCN1 channels (used for high-throughput screening assay development)
Comparison
Membrane potential-sensitive dye assay in… vs IonWorks HT electrophysiology platform
Design
Preclinical
Authors
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Supports high-throughput HCN modulator screening; leaves open clinical translation.
Effect estimate: r2 = 0.56
A novel, high-throughput MPSD assay for HCN channel blockers demonstrates acceptable reliability and moderate correlation with standard electrophysiology, facilitating future drug discovery.
Vasilyev et al. (2009) studied this question. Membrane potential-sensitive dye (MPSD) assay vs. IonWorks HT electrophysiology platform was evaluated on Correlation between MPSD and electrophysiology assay IC50s (r2 = 0.56). A novel membrane potential-sensitive dye assay for HCN channel modulators demonstrated low signal variability (Z' = 0.73) and moderate correlation with electrophysiology (r2 = 0.56).
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