Key result
Avian reovirus strain 2408 drives greater IL-6, IFN-γ, IL-10, and iNOS upregulation than strain S1133.
Infection with ARV strain 2408 induces higher levels of IL-6 and IFN-γ mRNA expression compared to strain S1133, which correlates with viral RNA levels and suggests a role in pathogenesis.
Strain 2408 may drive stronger inflammatory responses than S1133; hypothesis-generating for pathogenesis differences, requiring validation before any practice implications.
This study was undertaken to elucidate the cytokine response in chicken infected with strains of avian reovirus (ARV) S1133 and 2408. The expression levels of cytokine mRNA in the spleen and viral S1 RNA in various tissues at 1.5 and 2.5 days post inoculation (dpi) were examined using real-time quantitative PCR. Among the cytokines examined, the mRNA expression levels of IL-6, IFN-γ, IL-10 and iNOS at 2.5 dpi were significantly upregulated and higher in chickens infected with strain 2408 than in chickens infected with strain S1133, particularly IL-6 and IFN-γ. A significantly higher levels of viral S1 RNA were detected in the examined tissues from chickens infected with strain 2408 than with strain S1133 over the experimental course, among which the foot pad and spleen were more predominant. The highest levels of IL-6 and IFN-γ mRNA expression correlated with the viral S1 RNA levels in the spleen and the marked clinical diseases and gross lesions, suggesting that IL-6 and IFN-γ may play a role in the pathogenesis of ARV infection.
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Shen et al. (2014) studied Avian reovirus (ARV) infection. Avian reovirus strain 2408 vs. Avian reovirus strain S1133 was evaluated on Cytokine mRNA expression levels (IL-6, IFN-γ, IL-10, iNOS) and viral S1 RNA levels. Infection with avian reovirus strain 2408 resulted in significantly higher upregulation of IL-6, IFN-γ, IL-10, and iNOS mRNA expression at 2.5 days post inoculation compared to strain S1133.
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