Further Observations on the Kinetics of Hæmolysis, by Brilliant Green and Serum

A reinvestigation of the kinetics of brilliant green‐cell‐plasma systems, by methods in which the times for lysis are observed over a much greater range than heretofore, leads to the following conclusions:— 1. If the quantity of sensitising agent is constant and sufficient to produce sensitisation, the subsequently added plasma (or serum) appears to behave as a simple hæmolysin, for the time‐dilution curves obtained are typical over the greater part of their range. 2. The way in which the quantity of lysin (plasma) required to bring about lysis varies with the amount of sensitising agent used is very similar to the way in which the quantity of complement required to produce lysis in amboceptor‐cell‐complement systems varies with the amount of amboceptor used. 3. The same anomaly, viz. that unexpectedly large quantities of lysin are required when very large amounts of sensitising agents are employed, is met with both in brilliant green‐cell‐plasma systems and in amboceptor‐cell‐complement systems. 4. These results lead to the tentative conclusion that the kinetics of hæmolysis in brilliant green‐cell‐plasma systems are in general similar to those in amboceptor‐cell‐complement systems.